Journal Publications and Book Chapters

Representative publications:

  • Adriana San Miguel, Peri Kurshan, Mathew M. Crane, Patrick T. McGrath, Kang Shen, and Hang Lu+, “Deep phenotyping unveils hidden traits and genetic relations in subtle mutants”, Nature Communications, 7, Article number: 12990 (2016), doi:10.1038/ncomms12990. [Link]
  • Matthew M. Crane, Jeffrey N. Stirman, Chan-Yen Ou, Peri T. Kurshan, James M. Rehg, Kang Shen, and Hang Lu, “Autonomous screening implicates new genes in synaptogenesis in C. elegans”,  Nature Methods, 9, 977–980  doi:10.1038/nmeth.2141  [link] Highlighted by Nature Methods as one of the methods to watch
  • Kwanghun Chung*, Catherine A. Rivet*, Melissa L. Kemp, and Hang Lu, “Imaging single-cell signaling dynamics with a deterministic high-density single-cell trap array”, Analytical Chemistry,  2011, 83 (18), pp 7044–7052, DOI: 10.1021/ac2011153. [Link]
  • Jeffrey N. Stirman, Matthew M. Crane, Steven J. Husson, Christian Schultheis, Alexander Gottschalk, and Hang Lu, “Real-time multimodal optical control of individual neurons and muscles in freely behaving Caenorhabditis elegans”, Nature Methods, 2011, 8(2):153-8, doi:10.1038/nmeth.1555. [Link] [Nature Methods News and Views] [press release].
  • Kwanghun Chung*, Yoosik Kim*, Jitendra S. Kanodia, Emily Gong, Stanislav Y. Shvartsman, Hang Lu, “Large-scale microfluidic array for ordering, orienting and trapping of embryos”, Nature Methods, 2011, 8(2):171-6, doi:10.1038/nmeth.1548.   [Link] [Press release][Journal Cover]
  • Kwanghun Chung*, Matthew M. Crane*, and Hang Lu, “Automated on-chip rapid microscopy, phenotyping and sorting of C. elegans“. Nature Methods, 2008, 5, 637-643. [PDF] [Supplemental Materials and Methods] [Supplemental Movie1] [Supplemental Movie2[Supplemental Movie3] [Supplemental Movie4] [Supplemental Movie5] [Supplemental Movie6] [Supplemental Movie7] [Supplemental Movie8]Nature Methods News and Views [PDF]New York Times news articleGeorgia Tech news release.

All publications:

  1. Seleipiri Charles, Guillaume Aubry, Han-Ting Chou, Annalise B. Paaby, and Hang Lu, “High-temporal-resolution” smFISH method for gene expression studies in Caenorhabditis elegans embryos”, ACS Analytical Chemistry, 2021, 93, 3, 1369–1376
  2. Jason Wan and Hang Lu, “Enabling high-throuput single-animal gene expression studies with molecular and micro-scale technologies”, Lab on a Chip, 2020, 20, 4528-4538
  3. Fobang Liu, Josh Whitley, Nga Lee Ng, and Hang Lu, “Time‐resolved single‐cell assay for measuring intracellular reactive oxygen species upon exposure to ambient particulate matter”, Environmental Science & Technology, 2020, in press
  4. Emily L. Jackson-Holmes, Amanda W. Schaefer, Todd C. McDevitt, and Hang Lu, “Microfluidic perfusion modulates growth and motor neuron differentiation of stem cell aggregates”, Analyst, 2020, 145, 4815-4826. DOI: 10.1039/D0AN00491J
  5. Kim N. Le*, Mei Zhan*, Yongmin Cho*, Jason Wan, Dhaval S. Patel, and Hang Lu+, “HeALTH: An Automated Platform for Long-term Longitudinal Studies of Whole Organisms under Precise Environmental Control”, Communications Bio, 2020; 3 (1), 1-13
  6. Yi-Quan Tang, Sol Ah Lee, Mizanur Rahman, Siva A Vanapalli, Hang Lu, William R Schafer, “Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels”, Neuron, 107(1), 112-125.e10.
  7. Nicholas D. Testa, Samiksha Kaul, Mei Zhan, Kim N. Le, Hang Lu, Annalise B. Paaby+, “A portable, low-cost device for precise control of specimen temperature under stereomicroscopes”, PLoS One, 2020 Mar 11;15(3):e0230241. doi: 10.1371/journal.pone.0230241. eCollection 2020
  8. Yuehui Zhao, Lijiang Long, Jason Wan, Shweta Biliya, Shannon C. Brady, Daehan Lee, Akinade Ojemakinde, Erik C. Andersen, Fredrik O. Vannberg, Hang Lu, and Patrick T. McGrath+, “A spontaneous complex structural variant in rcan-1 increases exploratory behavior and laboratory fitness of Caenorhabditis elegans”. PLoS Genet 16(2): e1008606.
  9. Yongmin Cho, Sol Ah Lee, Yee Lian Chew, Kirby Broderick, William R. Schafer, and Hang Lu, “Multimodal Stimulation in a Microfluidic Device Facilitates Studies of Interneurons in Sensory Integration in C. elegans”, Small, 16 (10), 1905852, 2020
  10. Jason Wan, Gongchen Sun, Jocelyn Dicent, Dhaval S. Patel, and Hang Lu+, “smFISH in chips: a microfluidic-based pipeline to quantify in situ gene expression in whole organisms”, Lab on a Chip, 20, 266-273, 2020, DOI: 10.1039/C9LC00896A
  11. Gongchen Sun*, Jason Wan*, and Hang Lu+, “Rapid and Multi-cycle smFISH Enabled by Microfluidic Ion Concentration Polarization for In-situ Profiling of Tissue-specific Gene Expression in Whole C. elegans”, Biomicrofluidics, 13, 064101 (2019)
  12. Li Tao, Daniel Porto, Zhaoyu Li, Sylvia Fechner, Sol Ah Lee, Miriam B. Goodman, X.Z. Shawn Xu, Hang Lu, Kang Shen+, “Parallel processing of two mechanosensory modalities by a single neuron in C. elegans”, Developmental Cell, 51 (5), 617-, 2019, DOI: 10.1016/j.devcel.2019.10.008
  13. Luye He, Hyundoo Hwang, Melissa L. Kemp, and Hang Lu+, “Dynamic mitochondrial migratory features associated with calcium responses during T-cell antigen recognition”, J. of Immunology, 2019 Aug 1;203(3):760-768. doi: 10.4049/jimmunol.1800299. Epub 2019 Jun 14
  14. Dhaval S. Patel, Nan Xu, Hang Lu+, “Digging deeper: methodologies for high-content phenotyping in Caenorhabditis elegans”, Lab Animal, 48 (7), 207-216, 2019
  15. Kathleen Bates, Shen Jiang, Shivesh Chaudhary, Emily Jackson-Holmes, Melinda Jue, Erin McCaskey, Daniel Goldman, Hang Lu+, “Fast, versatile, and quantitative annotation of complex images”, BioTechniques, 66.6 (2019): 269-275.
  16. Yunfeng Chen, Lining Arnold Ju, Fangyuan Zhou, Jiexi Liao, Lingzhou Xue, Qian Peter Su, Dayong Jin, Yuping Yuan, Hang Lu, Shaun P. Jackson+, Cheng Zhu+, “An integrin αIIbβ3 intermediate affinity state mediates biomechanical platelet aggregation”, Nature Materials, 2019, 18, 760–769.
  17. Daniel A. Porto, John Giblin, Yiran Zhao, Hang Lu+, “Reverse-Correlation Analysis of Mechanosensation Circuit in C. elegans Reveals Temporal and Spatial Encoding”, Scientific Reports, 9.1 (2019): 5182.
  18. Gongchen Sun, and Hang Lu+, “Recent Advances in Microfluidic Techniques for Systems Biology”, Analytical Chemistry, 2019, 91 (1), 315-329. doi: 10.1021/acs.analchem.8b04757
  19. Fangyuan Zhou, Yunfeng Chen, Eric I. Felner, Cheng Zhu, and Hang Lu+, “Microfluidic auto-alignment of protein patterns for dissecting multi-receptor crosstalk in platelets”, Lab on a Chip, 2018, 18, 2966-2974
  20. Ivan de Carlos Cáceres, Daniel A. Porto, Ivan Gallotta, Pamela Santonicola, Josue Rodríguez-Cordero, Elia Di Schiavi, and Hang Lu+, “Automated Screening of C. elegans Neurodegeneration Mutants Enabled by Microfluidics and Image Analysis Algorithms”, Integrative Biology, 2018, 10 (9), 539-548, DOI: 10.1039/C8IB00091C
  21. Yee Lian Chew, Yoshinori Tanizawa, Yongmin Cho, Buyun Zhao, Alex J. Yu, Evan L. Ardiel, Ithai Rabinowitch, Jihong Bai, Catharine H. Rankin, Hang Lu, Isabel Beets, and William R. Schafer+, “An Afferent Neuropeptide System Transmits Mechanosensory Signals Triggering Sensitization and Arousal in C. elegans”, Neuron, 2018, 99 (6), 1233-1246, DOI: 10.1016/j.neuron.2018.08.003
  22. Farhan Kamili, and Hang Lu, “Recent advances and trends in microfluidic platforms for C. elegans biological assays”, Annual Review of Analytical Chemistry, in press.
  23. Yongmin Cho, David N. Oakland, Sol Ah Lee, William R. Schafer, and Hang Lu, “On-chip functional neuroimaging to mechanical stimulation in Caenorhabditis elegans larvae for studying functional role of neural circuits”, Lab on a Chip, 2018, 18, 601-609. DOI: 10.1039/C7LC01201B
  24. Tel Rouse, Guillaume Aubry, Yongmin Cho, Manuel Zimmer, and Hang Lu, “A programmable platform for sub-second multichemical dynamic stimulation and neuronal functional imaging in C. elegans”, Lab on a Chip, 2018 Jan 30;18(3):505-513. doi: 10.1039/c7lc01116d.
  25. Guillaume Aubry, and Hang Lu, “Droplet array for screening acute behaviour response to chemicals in Caenorhabditis elegans.” Lab on a Chip 17, no. 24 (2017): 4303-4311.
  26. Paul Villoutreix, Joakim Andén, Bomyi Lim, Hang Lu, Ioannis G Kevrekidis, Amit Singer, Stanislav Y Shvartsman+, “Synthesizing developmental trajectories”, PLoS computational Biology, 2017, 13, no. 9 (2017): e1005742. [Link]
  27. Dhaval S. Patel, Giovanni Diana, Eugeni V. Entchev, Mei Zhan, Hang Lu, QueeLim Ch’ng+, “Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans”, JoVE 126, 2017
  28. Emily L Jackson, Todd C McDevitt, Hang Lu+, “A microfluidic platform for physically isolated culture and multi-modal phenotypic analysis of stem cell aggregates”, Lab on a Chip, 2017,17, 3634-3642, DOI:10.1039/C7LC00763A.
  29. Yongmin Cho, Daniel A. Porto, Hyundoo Hwang, Laura J. Grundy, William R. Schafer, Hang Lu+, “Automated and controlled mechanical stimulation and functional imaging in vivo in C. elegans”, submitted, Lab on a Chip, 2017, 17, 2609-2618, DOI: 10.1039/C7LC00465F.
  30. Yogesh Goyal, Thomas J. Levario, Henry Mattingly, Susan Holmes, Stanislav Y. Shvartsman, and Hang Lu, “Parallel imaging of Drosophila embryos for quantitative analysis of genetic perturbations of the Ras pathway”, Disease Models & Mechanisms, 2017: doi: 10.1242/dmm.030163.
  31. Weipeng Zhuo, Hang Lu+, and Patrick T. McGrath+, “Microfluidic Platform with Spatiotemporally Controlled Micro-Environment for Studying Long-term C. elegans Developmental Arrests”, accepted, Lab on a Chip, 2017, 17, 1826-1833. DOI: 10.1039/C6LC01573E
  32. Yohei Matsunaga, Hyundoo Hwang, Barbara Franke, Rhys Willliams, McKenna Penley, Hiroshi Qadota, Hong Yi, Levi T. Morran, Hang Lu, Olga Mayans, and Guy Benian+, “Twitchin Kinase Inhibits Muscle Activity”, Molecular Biology of the Cell, 28, no. 12 (2017): 1591-1600.
  33. Ariel S. Kniss-James, Catherine A. Rivet, Loice Chingozha, Hang Lu, and Melissa L. Kemp, “Single-cell resolution of intracellular T cell Ca 2+ dynamics in response to frequency-based H2 O2 stimulation”, Integrative Biology, 2017, 9 (3), 238-247.
  34. Giovanni Diana, Dhaval S. Patel, Eugeni v. Entchev, Mei Zhan, hang Lu, and QueeLim Ch’ng, “Genetic Control of Encoding Strategy in a Food-sensing Neural Circuit”, eLife, accepted.
  35. Yongmin Cho, Charles L. Zhao, and Hang Lu, “Trends in High-throughput and Functional Neuroimaging in C. elegans”, WIREs Systems Biology and Medicine, accepted.
  36. Adriana San Miguel, Peri Kurshan, Mathew M. Crane, Patrick T. McGrath, Kang Shen, and Hang Lu+, “Deep phenotyping unveils hidden traits and genetic relations in subtle mutants”, Nature Communications, 7, Article number: 12990 (2016), doi:10.1038/ncomms12990. [Link]
  37. Catherine A. Rivet, Ariel S. Kniss-James, Margaret A. Gran, Anish Potnis, Abby Hill, Hang Lu, Melissa L. Kemp+, “Calcium Dynamics of Ex Vivo Long-Term Cultured CD8+ T Cells Are Regulated by Changes in Redox Metabolism”, PLoS One. 2016 Aug 15;11(8):e0159248. doi: 10.1371/journal.pone.0159248. eCollection 2016.
  38. Shinsuke Niwa, David M Lipton, Manatsu Morikawa, Charles Zhao, Nobutaka Hirokawa, Hang Lu, Kang Shen+, “Autoinhibition of a Neuronal Kinesin UNC-104/KIF1A Regulates the Size and Density of Synapses”, Cell Reports 2016 Aug 10. pii: S2211-1247(16)30961-5. doi: 10.1016/j.celrep.2016.07.043.
  39. Yi Liu and Hang Lu, “Microfluidics in systems biology-hype or truly useful?”, Current Opinions of Biotechnology, 2016 Jun;39:215-20. doi: 10.1016/j.copbio.2016.04.020.  PMID: 27267565 PMCID: PMC4901307 [Available on 2017-06-01]
  40. Emily L. Jackson and Hang Lu, “Three-dimensional models for studying development and disease: from organisms to organs-on-a-chip and organoids”, Integrative Biology, 2016 Jun 13;8(6):672-83. doi: 10.1039/c6ib00039h. Epub 2016 May 9. PMID: 27156572 PMCID: PMC4905804 [Available on 2017-06-13]
  41. Kathleen E. Bates, Hang Lu, “Optics-Integrated Microfluidic Platforms for Biomolecular Analyses”, Biophysical J., 2016 Apr 26;110(8):1684-97. doi: 10.1016/j.bpj.2016.03.018., PMID: 27119629 PMCID: PMC4850344 [Available on 2017-04-26]
  42. Thomas J. Levario, Charles Zhao, Stanislav Y. Shvartsman, and Hang Lu+, “Large-scale data collection and precise perturbation of live Drosophila embryos via a microfluidic platform”, Scientific Reports, 2016 Feb 11;6:21366. doi: 10.1038/srep21366, PMID: 26864815 PMCID: PMC4750044
  43. Dawn E. Barnes, Hyundoo Hwang, Kanako Ono, Hang Lu, and Shoichiro Ono+, “Molecular evolution of troponin I and a role of its N-terminal extension in nematode locomotion” Cytoskeleton, 2016 Mar;73(3):117-30. doi: 10.1002/cm.21281,PMID: 26849746 PMCID: PMC4846289 [Available on 2017-03-01]
  44. Hyundoo Hwang, Dawn E. Barnes, Yohei Matsunaga, Guy M. Benian, Shoichiro Ono, Hang Lu+, “Muscle contraction phenotypic analysis enabled by optogenetics reveals functional relationships of sarcomere components in Caenorhabditis elegans”, Scientific Reports, 2016 Jan 29;6:19900. doi: 10.1038/srep19900.  PMID: 26822332 PMCID: PMC4731793
  45. Thomas J. Levario, Bomyi Lim, Stanislav Y. Shvartsman, Hang Lu+, “Microfluidics for High-Throughput Quantitative Studies of Early Development”, Annual Review of Biomedical Engineering, 2016 Jul 11;18:285-309. doi: 10.1146/annurev-bioeng-100515-013926. Epub 2016 Feb 29.
  46. Md. Abul Hassan Samee, Bomyi Lim, Núria Samper, Hang Lu, Christine A. Rushlow, Gerardo Jiménez, Stanislav Y. Shvartsman, and Saurabh Sinha+, “A Systematic Ensemble Approach to Thermodynamic Modeling of Gene Expression from Sequence Data”, Cell Systems, 2015 Dec 23;1(6):396-407. doi: 10.1016/j.cels.2015.12.002. Epub 2015 Dec 23.
  47. Douglas E. White, Jonathan B. Sylvester, Melissa A. Kinney, Thomas J. Levario, Hang Lu, J. Todd Streelman, Todd C. McDevitt, Melissa L. Kemp+, “Quantitative Multivariate Analysis of Dynamic Multicellular Morphogenic Trajectories”, Integrative Biology, 2015 Jul 6;7(7):825-33. doi: 10.1039/c5ib00072f.
  48. Lining Ju, Yunfeng Chen, Fangyuan Zhou, Hang Lu, Miguel A. Cruz, and Cheng Zhu+, “Von Villebrand factor-A1 domain binds platelet glycoprotein Ibα in multiple states with distinctive force-dependent dissociation kinetics”, Thrombosis Research, 2015 Jun 20. pii: S0049-3848(15)30029-3. doi: 10.1016/j.thromres.2015.06.019.
  49. Bomyi Lim, Carmeline J. Dsilva, Thomas J. Levario, Hang Lu, Trudi Schüpbach, Ioannis G. Kevrekidis, and Stanislav Y. Shvartsman+, “Dynamics of Inductive ERK Signaling in the Drosophila Embryo”, Current Biology, 2015 Jun 29;25(13):1784-90. doi: 10.1016/j.cub.2015.05.039. Epub 2015 Jun 18.
  50. Robert J. Mallis, Ke Bai, Haribabu Arthanari, Rebecca E. Hussey, Maris Handley, Zhenhai Li, Loice Chingozha, Jonathan S. Duke-Cohan, Hang Lu, Jia-Huai Wang, Cheng Zhu, Gerhard Wagner, Ellis L. Reinherz+, “Pre-T cell receptor ligand binding impacts thymocyte development prior to αβ TCR expression”, PNAS, 2015 Jul 7;112(27):8373-8. doi: 10.1073/pnas.1504971112. Epub 2015 Jun 8. PMCID: PMC4500245
  51. Mei Zhan, Matthew M. Crane, Eugeni Entchev, Antonio Caballero, Diana Andrea Fernandes de Abreu, QueeLim Ch’ng, and Hang Lu+ “Automated Processing of Imaging Data Through Multi-Tiered Classification of Biological Structures Illustrated Using Caenorhabditis elegans”, PLoS Computational Biology, April 24, 2015, DOI: 10.1371/journal.pcbi.1004194. PMCID: PMC4409145
  52. Eugeni V. Entchev*, Dhaval S. Patel*, Mei Zhan*, Andrew J. Steele, Hang Lu+, and QueeLim Ch’ng+, “A gene-expression-based neural code for food abundance that modulates lifespan”, eLife, 2015;4:e06259. DOI: PMCID: PMC4417936
  53. Carmeline J Dsilva, Bomyi Lim, Hang Lu, Amit Singer, Ioannis G Kevrekidis, and Stanislav Y Shvartsman+, “Temporal ordering and registration of images in studies of developmental dynamics”, Development, 142:1717-1724, 2015.  PMCID: PMC4419277
  54. Guillaume Aubry, Mei Zhan, and Hang Lu+, “Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans”, Lab on a Chip, 2015, 15, 1424 – 1431, DOI: 10.1039/C4LC01384K. Also a cover art of the issue. PMCID PMC4348330
  55. Luye He,* Ariel Kniss,* Adriana San-Miguel, Tel Rouse, Melissa L. Kemp, Hang Lu, “An automated platform enabling dynamic stimuli delivery and cellular response readout for high-throughput single-cell signaling studies”, Lab on a Chip, 2015, 15, 1497 – 1507, DOI: 10.1039/C4LC01070A.
  56. Loice Chingozha, Mei Zhan, Cheng Zhu, Hang Lu, “A generalizable, tunable microfluidic platform for delivering fast temporally varying chemical signals to probe single-cell response dynamics”, Analytical Chemistry, 86 (20), pp 10138–10147, DOI: 10.1021/ac5019843.
  57. Hyewon Lee, Shin Ae Kim, Paula Mugno, Marc Hammarlund, Massimo A. Hilliard, and Hang Lu, “A Multi-channel device for high-density target-selective stimulation and long-term monitoring of cells and subcellular features in C. elegans”, Lab on a Chip, 2014,14, 4513-4522. DOI: 10.1039/C4LC00789A
  58. Peri T. Kurshan, Allan Q. Phan, George J. Wang, Matthew M. Crane, Hang Lu, Kang Shen, “Regulation of synaptic extracellular matrix composition is critical for proper synapse morphology”, J. Neurosci. 2014, 34(38): 12678-12689
  59. Hyundoo Hwang, Jan Krajniak, Yohei Matsunaga, Guy Benian, and Hang Lu, “On-demand optical immobilization of Caenorhabditis elegans for high-resolution imaging and microinjection”, Lab on a Chip, 2014, 14 (18), 3498 – 3501. PMCID: PMC4148454
  60. Xiaoni Ai*, Weipeng Zhuo*, Qionglin Liang, Patrick T. McGrath, and Hang Lu, “A high-throughput device for size based separation of C. elegans developmental stages”, Lab on a Chip, 2014, 14(10):1746-52. doi: 10.1039/c3lc51334c.  PMCID: PMC4042318.
  61. Guillaume Aubry and Hang Lu, “A perspective on optical developments in microfluidic platforms for Caenorhabditis elegans research”, Biomicrofluidics, 2014, 8(1):011301. doi: 10.1063/1.4865167. PMCID: PMC3977797
  62. Diana Andrea Fernandes de Abreu, Antonio Caballero, Pascal Fardel, Nicholas Stroustrup, Zhunan Chen, KyungHwa Lee, William D. Keyes, Zachary M. Nash, Isaac F. López Moyado, Federico Vaggi, Astrid Cornils, Martin Regenass, Anca Neagu, Ivan Ostojic, Chang Liu, Yongmin Cho, Deniz Sifoglu, Walter Fontana, Hang Lu, Attila Csikasz-Nagy, Coleen Murphy, Adam Antebi, Eric Blanc, Javier Apfeld, Yun Zhang, Joy Alcedo, QueeLim Ch’ng, “An Insulin-to-Insulin Regulatory Network Orchestrates Phenotypic Specificity in Development and Physiology”, PLoS Genetics, 2014,10(3):e1004225. doi: 10.1371/journal.pgen.1004225.  PMCID: PMC3967928
  63. Devon M. Headen, Guillaume Aubry, Hang Lu, and Andres Garcia, “Microfluidic-Based Generation of Size-Controlled, Biofunctionalized Synthetic Polymer Microgels for Cell Encapsulation”, Advanced Materials, 2014, 26(19):3003-8. doi: 10.1002/adma.201304880. PMCID: PMC4058833
  64. Torri E. Rinker*, Taymour M. Hammoudi*, Melissa L. Kemp, Hang Lu, Johnna S. Temenoff, “Interactions between Mesenchymal Stem Cells, Adipocytes, and Osteoblasts in a 3D Tri-Culture Model of Hyperglycemic Conditions in the Bone Marrow Microenvironment”, Integrative Biology, 2014, 6(3):324-37. doi: 10.1039/c3ib40194d. PMCID: PMC3965183
  65. Celine I. Maeder, Adriana San Miguel, Hang Lu, Kang Shen, “In vivo neuron-wide analysis of synaptic vesicle precursor trafficking”, Traffic, 2014, 15(3), 273-291. doi: 10.1111/tra.12142. PMCID:
  66. Jeffrey N. Stirman, Bethany Harker, Hang Lu, Matthew Crane, “Animal microsurgery using microfluidics”, Current Opinion in Biotechnology, 2014, 25: 24-29. doi: 10.1016/j.copbio.2013.08.007. PMCID: PMC3912467
  67. Jenna L. Wilson, Shalu Suri, Ankur Singh, Catherine A. Rivet, Hang Lu, Todd C. McDevitt, “Single-cell Analysis of Embryoid Body Heterogeneity Using Microfluidic Trapping Array” Biomedical Microdevices, 2014, 16(1):79-90. doi: 10.1007/s10544-013-9807-3. PMCID: PMC3945678.
  68. Emily L. Jackson, Hang Lu, “Advances in microfluidic cell separation and manipulation”, Current Opinions in Chemical Engineering, 2013, 2(4):398-404. PMCID: PMC3970816
  69. Shalu Suri, Ankur Singh, Anh H. Nguyen, Andres M. Bratt-Leal, Todd C. McDevitt and Hang Lu “Microfluidic-based patterning of embryonic stem cells for in vitro development studies”, Lab on a Chip, 2013, 13(23):4617-24. doi: 10.1039/c3lc50663k.
  70. Daniel C. Williams, Rachid El Bejjani, Paula Mugno Ramirez, Sean Coakley, Shinae Kim, Hyewon Lee, Quan Wen, Aravi Samuel, Hang Lu, Massimo A. Hilliard, Marc Hammarlund, “Rapid and permanent neuronal inactivation in vivo via subcellular generation of reactive oxygen with the use of KillerRed”, Cell Reports, 2013 Oct 31;5(2):553-63. doi: 10.1016/j.celrep.2013.09.023.
  71. Mei Zhan, Loice Chingozha, and Hang Lu, “Enabling Systems Biology Approaches Through Microfabricated Systems” Analytical Chemistry, 2013 Oct 1;85(19):8882-94. doi: 10.1021/ac401472y; also a cover article.
  72. Adriana San-Miguel and Hang Lu, invited, “Microfluidics as a Tool for C. elegans Biological Research”, Wormbook, 2013 Sep 24:1-19. doi: 10.1895/wormbook.1.162.1.
  73. Bomyi Lim, Nuria Samper, Hang Lu, Christine Rushlow, Gerardo Jimenez, and Stanislav Y. Shvartsman, “Kinetics of gene derepression by ERK signaling”, PNAS, 2013 Jun 18;110(25):10330-5. doi: 10.1073/pnas.1303635110. Epub 2013 Jun 3.
  74. Ariel Kniss, Hang Lu, Dean P. Jones, Melissa L. Kemp, “A microfluidic systems biology approach for live single-cell mitochondrial ROS imaging”, 2013, Methods in Enzymology, 526: 219-230, doi: 10.1016/B978-0-12-405883-5.00013-2.
  75. Robin W. Klemm, Justin P. Norton, Ronald A. Cole, Chen S. Li, Matthew M. Crane, Liying Li, Diana Jin, Alexandra Boye-Doe, Yoko Shibata, Hang Lu, Robert V. Farese Jr., Tom Rapoport, Craig Blackstone, Yi Guo, and Ho Yi Mak, “a conserved role for atlastin GTPases in regulating lipid droplet size”, Cell Reports, 2013 May 15. pii: S2211-1247(13)00197-6. doi: 10.1016/j.celrep.2013.04.015.
  76. Jan Krajniak, Yan Hao, Ho Yi Mak, Hang Lu, “CLIP – Continuous Live Imaging Platform for Direct Observation of C. elegans Physiological Processes”, Lab on a Chip, 2013, 13 (15), 2963 – 2971. DOI:10.1039/C3LC50300C
  77. Ankur Singh, Shalu Suri, Ted Lee, Jamie M.Chilton, Weiqiang Chen,  Jianping Fu, Steven L. Stice, Hang Lu, Todd C. McDevitt, and Andrés J. García, “Adhesion strength-based, label-free isolation of human pluripotent stem cells”, Nature Methods, 2013 May;10(5):438-44. doi: 10.1038/nmeth.2437. Epub 2013 Apr 7.
  78. Thomas J Levario, Mei Zhan, Bomyi Lim, Stanislav Y Shvartsman, and Hang Lu, “Microfluidic Trap Array for Massively Parallel Imaging of Drosophila Embryos”, Nature Protocols, 2013 Apr;8(4):721-36. doi: 10.1038/nprot.2013.034. Epub 2013 Mar 14.
  79. Sharon K. Hamilton, Nathaniel C. Bloodworth, Christopher S. Massad, Taymour M. Hammoudi, Shalu Suri, Peter J. Yang, Hang Lu, and Johnna S. Temenoff, “Development of 3D hydrogel culture systems with on-demand cell separation”, Biotechnology J., 2013 Apr;8(4):485-95. doi: 10.1002/biot.201200200. Epub 2013 Feb 28.
  80. Edward S. Park, Michael A. DiFeo, Jacqueline M. Rand, Matthew M. Crane and Hang Lu, “Sequentially-pulsed fluid delivery to establish soluble gradients within a scalable microfluidic chamber array”, Biomicrofluidics, 2013, 7: 011804, doi: 10.1063/1.4774313.
  81. Hyundoo Hwang, and Hang Lu, “Microfluidic tools for developmental studies of small model organisms –nematodes, fruit flies, and zebrafish”, invited review for Biotechnology Journal, 2013 Feb;8(2):192-205. doi: 10.1002/biot.201200129. Epub 2012 Nov 19.
  82. Hyewon Lee, Matthew M. Crane, Yun Zhang, and Hang Lu, “Quantitative screening of genes regulating tryptophan hydroxylase transcription in C. elegans using microfluidics and adaptive algorithm”, Integrative Biology, 2013, 5 (2), 372 – 380. doi: 10.1039/c2ib20078c.
  83. Mei Zhan, Hang Lu, “Quantitative Evo-Devo in Microfluidic Devices”, Advances in Evolution and Development, edited by J. Todd Streelman, John Wiley & Sons, 2013.
  84. Matthew M. Crane, Jeffrey N. Stirman, Chan-Yen Ou, Peri T. Kurshan, James M. Rehg, Kang Shen, and Hang Lu, “Autonomous screening implicates new genes in synaptogenesis in C. elegans”,  Nature Methods, 9, 977–980  doi:10.1038/nmeth.2141  [link] Highlighted by Nature Methods as one of the methods to watch
  85. Aharon Helman, Bomyi Lim, María José Andreu, Yoosik Kim, Tatyana Shestkin, Hang Lu, Gerardo Jiménez, Stanislav Y. Shvartsman, and Ze’ev Paroush, “RTK signaling modulates the Dorsal gradient”, accepted, Development.
  86. Kristin M. French, Archana V. Boopathy, Jessica A. DeQuach, Loice Chingozha, Hang Lu, Karen L. Christman, Michael E. Davis, “A Naturally-Derived Cardiac Extracellul ar Matrix Enhances Cardiac ProgenitorCell Behavior In Vitro”, accepted, Acta Biomaterialia. DOI:
  87. Steven Husson, Jana Liewald, Jeffrey Stirman, Hang Lu, and Alexander Gottschalk, “Microbial light-activatable proton pumps as circuit breakers to functionally dissect neuronal networks in C. elegans”, PLoS ONE, 2012 7(7): e40937. doi:10.1371/journal.pone.0040937
  88. Sharon B. Sann, Matthew M. Crane, Alicia Arney, Hang Lu, Yishi Jin, “Rabx-5 regulates Rab-5 early endosomal compartments and synaptic vesicle formation in C. elegans”, PLoS ONE, 2012 7(6): e37930. doi:10.1371/journal.pone.0037930.
  89. Taymour M. Hammoudi, Catherine A. Rivet, Melissa L. Kemp, Hang Lu, and Johnna S. Temenoff, “Three-Dimensional In Vitro Tri-Culture Platform to Investigate Effects of Crosstalk between Mesenchymal Stem Cells, Osteoblasts and Adipocytes”, Tissue Engineering, 2012. doi:10.1089/ten.tea.2011.0691
  90. Ivan de Carlos Cáceres, Nicholas Valmas, Massimo A. Hilliard, Hang Lu, ”Laterally Orienting C. elegans Using Geometry at Microscale for High-Throughput Visual Screens in Neurodegeneration and Neuronal Development Studies”, PLoS ONE, 2012, 7(4): e35037. doi:10.1371/journal.pone.0035037
  91. Steven J Husson, Wagner Steuer Costa, Jeffrey N Stirman, Joseph D Watson, W. Clay Spencer; Millet Treinin, David M Miller III, Hang Lu, Alexander Gottschalk, “Optogenetic analysis of a nociceptor neuron and network reveals ion channels acting downstream of primary sensors”, Current Biology, 2012 22 (9): 743-752, doi:10.1016/j.cub.2012.02.066
  92. Jitendra S. Kanodia, Hsiao-Lan Liang, Yoosik Kim, Bomyi Lim, Mei Zhan, Hang Lu, Christine A. Rushlow, and Stanislav Y. Shvartsman, “Pattern Formation by Graded and Uniform Signals in the Early Drosophila Embryo”, Biophysical Journal, 2012 102 (3): 427-433. doi:10.1016/j.bpj.2011.12.042.
  93. Jeffrey N. Stirman, Matthew M. Crane, Steven J. Husson, Alexander Gottschalk, and Hang Lu, “Assembly of a multispectral optical illumination system with precise spatiotemporal control for the manipulation of optogenetic reagents”, Nature Protocol, 2012 7(2):207-20, doi: 10.1038/nprot.2011.433.
  94. John F. Nahabedian, Hiroshi Qadota, Jeffrey N. Stirman, Hang Lu, Guy M. Benian, “Bending amplitude – A new quantitative assay of C. elegans locomotion: Identification of phenotypes for mutants in genes encoding muscle focal adhesion components”, Methods. 2011 Nov 22. [Epub ahead of print]
  95. Kwanghun Chung*, Mei Zhan*, Jagan Srinivasan, Paul W. Sternberg, Emily Gong, Frank Schroeder, and Hang Lu, “’Microfluidic chamber arrays for whole-organism behavior-based chemical screening”, in press, Lab Chip, 2011, DOI:10.1039/C1LC20400A
  96. Jitendra S Kanodia, Yoosik Kim, Raju Tomer, Zia Khan, Kwanghun Chung, John D Storey, Hang Lu, Philipp J Keller, and Stanislav Y Shvartsman, “A computational statistics approach for estimating the spatial range of morphogen gradients”, Development, 2011 Nov;138(22):4867-74.
  97. Kwanghun Chung*, Catherine A. Rivet*, Melissa L. Kemp, and Hang Lu, “Imaging single-cell signaling dynamics with a deterministic high-density single-cell trap array”, Analytical Chemistry,  2011, 83 (18), pp 7044–7052, DOI: 10.1021/ac2011153. [Link]
  98. Yoosik Kim, Maria Jose Andreu, Bomyi Lim, Kwanghun Chung, Mark Terayama, Gerardo Jimenez, Celeste A. Berg, Hang Lu, and Stanislav Y. Shvartsman, “Gene Regulation by MAPK Substrate Competition”, Developmental Cell, 2011, 20 (6): 880-887, doi:10.1016/j.devcel.2011.05.009.
  99. Jeffrey N. Stirman, Matthew M. Crane, Steven J. Husson, Christian Schultheis, Alexander Gottschalk, and Hang Lu, “Real-time multimodal optical control of individual neurons and muscles in freely behaving Caenorhabditis elegans”, Nature Methods, 2011, 8(2):153-8, doi:10.1038/nmeth.1555. [Link] [Nature Methods News and Views] [press release].
  100. Kwanghun Chung*, Yoosik Kim*, Jitendra S. Kanodia, Emily Gong, Stanislav Y. Shvartsman, Hang Lu, “Large-scale microfluidic array for ordering, orienting and trapping of embryos”, Nature Methods, 2011, 8(2):171-6, doi:10.1038/nmeth.1548.   [Link] [Press release][Journal Cover]
  101. Hiroshi Qadota, Takayuki Miyauchi, John F. Nahabedian, Jeffrey N. Stirman, Hang Lu, Mutsuki Amano, Guy M. Benian, and Kozo Kaibuchi, “PKN-1, a Homologue of Mammalian PKN, Is Involved in the Regulation of Muscle Contraction and Force Transmission in C. elegans”, J Mol Biol. 2011 Jan 26. [Epub ahead of print], doi:10.1016/j.jmb.2011.01.039.
  102. Catherine A. Rivet, Abby S. Hill, Hang Lu, Melissa L. Kemp, “Predicting cytotoxic T cell age from multivariate analysis of static and dynamic biomarkers”, in press, Molecular & Cellular Proteomics, 2011, 10 (3): mcp.M110.003921. First Published on December 30, 2010, doi:10.1074/mcp.M110.003921. [Link][Press release]
  103. Gary L. Moulder, Gina H. Cremona, Janet Duerr, Jeffrey N. Stirman, Stephen D. Fields, Wendy Martin, Hiroshi Qadota, Guy M. Benian, Hang Lu, and Robert J. Barstead, “α-actinin is Required for Proper Assembly of Z-disk / Focal Adhesion-Like Structures and for Efficient Locomotion in Caenorhabditis elegans”, J. Mol. Biol., 2010 Sep 15. [Epub ahead of print].
  104. Stephen J. Pety, Hang Lu, Yonathan S. Thio, “Microfluidics meets dilute solution viscometry: an undergraduate laboratory to determine polymer molecular weight using a microviscometer”, Chemical Engineering Education, 2010.
  105. Catherine Rivet*, Hyewon Lee*, Alison Hirsch*, and Sharon Hamilton*, Hang Lu “Microfluidics for Medical Diagnostics and Biosensors”, invited review in a Special Issue of Authoritative Reviews, Chemical Engineering Science, in press 2010.
  106. Jeffrey N. Stirman, Martin Brauner, Alexander Gottschalk, and Hang Lu, “Microfluidic system for high-throughput studies of synaptic functions using ChR2”, in press, J. Neurosci. Methods, 2010, 191 (1), 90-93, doi:10.1016/j.jneumeth.2010.05.019 [LINK]
  107. Taymour M. Hammoudi, Hang Lu, Johnna S. Temenoff, “Long-Term Spatially Defined  Co-Culture within Three-Dimensional Photopatterned Hydrogels: A Technical Note”, Tissue Engineering C,  16, 1621-1628, 2010. [LINK]
  108. Jan Krajniak and Hang Lu, “Long-term High-Resolution Imaging and Culture of C. elegans in Chip-Gel Hybrid Microfluidic Device for Developmental Studies”, Lab on a Chip DOI:10.1039/c001986k. [PDF]
  109. Matthew Crane, Kwanghun Chung, Jeffrey Stirman, Hang Lu, “Microfluidics-enabled phenotyping, imaging, and screening of multicellular organisms”, invited review for Lab on a Chip, 2010, 10, 1509 – 1517, DOI:10.1039/B927258E. [PDF]
  110. Edward S. Park, Ashley E. Carson, Michael A. DiFeo, Thomas H. Barker, and Hang Lu, “Continuously Perfused, Individually Isolated Microfluidic Chamber Array for Studying Cellular Responses to Orthogonal Combinations of Matrix and Soluble Signals”, Lab on a Chip, 2010, 10: 571-580, DOI: 10.1039/b919294h.  [PDF] [also a cover article]
  111. Sharon Hamilton, Hang Lu, and Johnna Temenoff, “Patterned Hydrogels for Tissue Engineering with Stem Cells”, to appear in Biomaterials as Stem Cell Niche, Ed. By Krishnendu Roy, in series “Studies in Mechanobiology, Tissue Engineering and Biomaterials” (Series Editor: Prof. Amit Gefen) by Springer-Verlag, 2010.
  112. Jan Krajniak, Edward Park, and Hang Lu, “Packaging and Integration of Microfluidic and bioMEMS Devices”, to appear in Nano and Bio Packaging, Ed. C.P. Wong, G.Y. Li, Springer, New York, 2009.
  113. Adela Ben-Yakar, Nikos Chronis, Hang Lu, “Microfluidic tools for the analysis of behavior and neural cell physiology in worms”, invited review for Current Opinion in Neurobiology, 2009, 19:561–567, doi:10.1016/j.conb.2009.10.010.
  114. Kwanghun Chung*, Hyewon Lee*, and Hang Lu, “Multiplex pressure measurement in microsystems using volume displacement of particle suspensions”, Lab on a Chip, 2009, 9: 3345 – 3353, DOI: 10.1039/b911480g. [PDF]
  115. Kwanghun Chung and Hang Lu, “Automated laser ablation of neurons in microfluidic system”, Lab on a Chip, 2009, 9, 2764 – 2766, DOI:10.1039/B910703G. [Link] [Hot Article] [Highlight in Chemical Technology][PDF]
  116. Kwanghun Chung*, Jaekyu Cho*, Edward Park, Victor Breedveld, and Hang Lu, “Three-dimensional In Situ Temperature Measurement in Microfluidic System Using Brownian Motion of Nanoparticles”, Analytical Chemistry, 2009, 81(1): 210-217.  DOI:10.1021/ac802031j. [PDF]
  117. Alison M. Hirsch, Catherine A. Rivet, Boyang Zhang, Melissa L. Kemp, and Hang Lu, “Parallel multi-time point cell stimulation and lysis on-chip for studying early signaling events in T cell activation”, Lab on a Chip, 2009, DOI:10.1039/B810896J. [PDF] [Hot Article]
  118. Matthew M. Crane, Kwanghun Chung, and Hang Lu. “Computer-enhanced high-throughput genetic screens of C. elegans in a microfluidic system”, Lab on a Chip, 2009, 9, 38-40. [PDF] [Supplemental Movie]
  119. Kwanghun Chung*, Matthew M. Crane*, and Hang Lu, “Automated on-chip rapid microscopy, phenotyping and sorting of C. elegans“. Nature Methods, 2008, 5, 637-643. [PDF] [Supplemental Materials and Methods] [Supplemental Movie1] [Supplemental Movie2] [Supplemental Movie3] [Supplemental Movie4] [Supplemental Movie5] [Supplemental Movie6] [Supplemental Movie7] [Supplemental Movie8]; Nature Methods News and Views [PDF]; New York Times news article; Georgia Tech news release.
  120. Yun Zhang, Hang Lu, and Cornelia I. Bargmann, “Pathogenic bacteria induce aversive olfactory learning in Caenorhabditis elegans”, Nature, 2005, 438 (7065): 179-184. [PDF] [Supplemental Materials]
  121. Jesse M. Gray*, David S. Karow*, Hang Lu, Andy J. Chang, Jennifer S. Chang, Ron Ellis, Michael A. Marletta, and Cornelia I. Bargmann, “Oxygen sensation and social feeding mediated by a C. elegans guanylate cyclase homologue”, Nature, 2004, 430 (6997): 317-322 [PDF] [Supplemental Materials]
  122. Hang Lu, Martin A. Schmidt, and Klavs F. Jensen, “Electroporation Microdevice for Controlled Cell Lysis”, Lab Chip, 2005; 5 (1), 23-29 [PDF]
  123. Hang Lu, Klavs F. Jensen, “Intracellular Analysis On-Chip”, Lab-on-a-chips for Cellomics: Micro and Nanotechnologies for Life Science, Ed. H. Andersson and A. van den Berg, invited.
  124. Hang Lu*, Lily Y. Koo*, W. Maria Wang, Douglas A. Lauffenburger, Linda G. Griffith, Klavs F. Jensen, “Microfluidic Shear Devices for Quantitative Analysis of Cell Adhesion”, Analytical Chemistry 2004; 76 (18), 5257-5264 [PDF]
  125. Hang Lu, Suzanne Gaudet, Martin A. Schmidt, and Klavs F. Jensen, “A Microfluidic Device for Subcellular Organelle Sorting”, Analytical Chemistry 2004; 76 (19), 5705-5712 [PDF]
  126. Joerg Lahann, Mercedes Balcells, Hang Lu, Teresa Rodon, Klavs F. Jensen, and Robert Langer, “Reactive Polymer Coatings: A First Step Towards Surface Engineering of Microfluidic Devices”, Analytical Chemistry 2003; 75 (9): 2117-2122
  127. Hang Lu, Martin A. Schmidt, and Klavs F. Jensen, “Photochemical Reactions and On-line UV Detection in Microfabricated Reactors”, Lab Chip 2001; 1 (1): 22 [PDF]

* equal authorship